a simplified and reproducible two-step method for the purification of prostate-specific antigen

نویسندگان

اسکندر کمالی سروستانی

eskandar kamali sarvestani عبدالعزیز خضری

abdolaziz khezri محمود وصال

mohamoud vessal عباس قادری

abbas ghaderi

چکیده

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five different isoforms of this protein. the immunoreactivity of the purified psa was checked by western blotting. this simple two-step method can be used for a large-scale preparation of the purified psa for the clinical tests and also for further investigative studies on the biological properties of this protein.

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A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen

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عنوان ژورنال:
iranian biomedical journal

جلد ۳، شماره ۳، صفحات ۸۹-۹۱

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